Abstract Information


Presenting Author(s) Mark Brown
Abstract Title Application of small scale expression analysis to the study of cell cycle regulation in mouse primary chondrocytes.
Full author List Mark Brown, Zenobia Ali, Phyllis LuValle
Text of abstract

Bone formation during vertebral development follows one of two general processes, intramembranous and endochondral ossification. During endochondral ossification, a cartilagenous precursor provides a scaffold for replacement by bone tissue in a tightly regulated temporal and spatial progression. Endochondral bone growth is controlled by the coordinated proliferation and differentiation of chondrocytes. Our lab and others have demonstrated that cell cycle proteins are implicated in the regulation of these processes but their exact roles remain to be elucidated. One cell-cycle protein which has been shown to be key in the regulation of chondrocyte proliferation in vitro is cyclin D1.

We have been applying a number of small scale expression techniques including chromatin immunoprecipition, subtractive hybridisation and differential PCR analysis to the study of cell cycle regulation in mouse primary chondrocytes in vitro. In this work, we demonstrate that Cyclin D2 and Cyclin D3 can compensate for loss of Cyclin D1 expression in knockout and knockdown mouse chondrocytes in vitro.

Which session is your work most relevant to: Tissue Differentiation