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Presenting Author(s) April DeLaurier
Abstract Title Developing 3-D imaging methods to characterise limb development: a comparison of forelimb and hind limb morphology in the mouse.
Full author List DeLaurier, A., Mohun, T., Bennett, M., Logan, M.
Text of abstract

The vertebrate limb is comprised of a number of structures, including bone, muscle and tendon that develop in a precise manner and form complex associations in three-dimensional space. The developing mouse limb is commonly used as a model for studying processes of tissue morphogenesis in transgenic and disease research; however, a comprehensive reference for the normal patterning of limb structures is lacking. Therefore, one aim of this project is to create a detailed 3-D reference model of the bone, muscle, and tendon of embryonic wild-type limbs as a resource for comparative studies. We are also using the same techniques to characterise the altered limb morphology of transgenic mouse lines.

We are using optical projection tomography (OPT), which allows the visualisation of tissues through a combination of 3-D modelling and reconstruction of "virtual" sections. Using OPT we have analysed muscle patterning by fluorescent immunohistochemical labelling in wild-type mouse embryonic forelimbs and hind limbs at stage E14.5. We are also developing methods for visualisation of tendon and skeletal elements in 3-D. Using OPT we have created virtual serial sections and interactive 3-D models of limbs which give the user control of the positioning and magnification of the specimen. This method has tremendous advantages over traditional 2-D microscopy, as it allows the study of limb structures from a variety of perspectives. The ultimate objective of this project is to make this data available as a permanent public resource available to the wider scientific community for comparative study of limb tissue patterning.
Which session is your work most relevant to: Limb patterning