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Presenting Author(s) Kenneth Lee
Abstract Title Proteomic Analysis of Mouse Embryonic Interdigital Tissues: Identification of Proteins Differentially Expressed during the Onset of Programmed Cell Death.
Full author List Kenneth Lee, SW Shan and MK Tang
Text of abstract The aim of this study was to identify proteins that were involved in the regulation of interdigital cell death. Proteomic techniques were used to establish the protein profiles of the embryonic day (E) 12.5 and E13.5 mouse hindlimb interdigital tissues. Mouse interdigital cells normally begin to die at E13.5. We established that protein disulfide isomerase 3 (PDI-3) was expressed at E12.5 and up-regulated at E13.5. In contrast, peroxiredoxins 1 (Prx-1) was expressed at E12.5 but down regulated at E13.5. Semi-quantitative RT-PCR and western blot analyses revealed that the expression patterns for PDI-3 and Prx-1 were the same as for the respective proteins in the 2-demensional electrophoresis gels. Tissue culture experiments showed that it was possible to up-regulate PDI-3 expression by manipulating the interdigital tissues to die during culture but not when the tissues were made to survive. Conversely, we determined that Prx-1 expression was maintained when interdigital cultures were manipulated to survive but down regulated when the cultures were allowed to die. These results suggest that Prx-1 is involved in maintaining interdigital cell survival while PDI-3 is involved in initiating interdigital cell death. We have also identified 11 more proteins that were differentially expressed in the interdigital tissues. In conclusion, interdigital cell death is not a simple process but involves factors that determine cell death and cell survival.
Which session is your work most relevant to: Tissue differentiation