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Presenting Author(s) Londale Madrigal
Abstract Title Rotation of the Mammalian Hindlimb.
Full author List Madrigal L., Ashby P., Tickle C., MacKenzie A. & Miedzybrodzka Z.
Text of abstract

Abnormal hindlimb development is seen in the human multifactorial disorder talipes equinovarus (clubfoot). In clubfoot the normal late embryological rotation of the foot from the vertical to the plantar plane is arrested. Little is known about the differences between normal hind- and forelimb development, and, in particular, about the later stages of hindlimb development. Indeed, even gross morphological changes taking place at these later stages remain ill described. In order to inform our studies of clubfoot aetiology, we wish to identify the genetic mechanisms controlling this stage of development. As a prelude to this work, we are describing in detail the late development of normal CD1 mice hindlimbs. CD1 embryos from stages E14.5 - E19.5 d.p.c. were harvested and fixed in 4 % PFA. Hindlimbs were observed using stereomicroscopy and / digital photography. Late hindlimb development comprises internal rotation and angulation. Rotation commenced at E15.5, but not before. In all 108 embryos analysed, rotation of one hindfoot was always completed before rotation of the second hindlimb commenced. In 68 embryos (63%), the right hindlimb rotated before the left (p = < 0.01, 1 d.f.). This predominance of rotation of the right hindlimb before the left is interesting as in clubfoot, the right foot is affected more often than the left.

Which session is your work most relevant to: Limb patterning/ Human limb abnormalities